ThermoPhage™ Lysozyme from Bacteriopage Ph2119

lys164

220,00

A highly thermostable Lysozyme developed from a unique thermophilic bacteriophage. 

 


SKU: lys164.
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Description

lys164A highly thermostable Lysozyme developed from a unique thermophilic bacteriophage. The enzyme breaks down the cell wall of specific bacteria and is especially active against cells of thermophilic bacterial strains of the genus Thermus including T. thermophilus and T. aquaticus.

 

The enzyme is available for purchase in 0.1 mg size.

Shipping within a week, shipping and handling charges additional to price listed below.

Product : Lys164 | ThermoPhage Lysozyme | Price : 220.00 EUR

 

For further information read more below or download the product sheet

 

Enzyme activity: ThermoPhage Ts Lysozyme promotes lysis of certain bacteria probably through hydrolysis of linkages between sugar residues and peptides of the cell wall peptidoglycan. 

Synonyms: muramidase, N-acetylmuramide glycanhydrolase, glycoside hydrolase

 

Enzyme classification: EC 3.5.1.28

 

Source: Thermophilic bacteriophage Ph2119

 

Description: ThermoPhageLysozyme originates from a thermophilic bacteriophage known to infect thermophilic bacteria of the genus Thermus. The enzyme promotes lysis of certain bacterial species but has activity spectrum very different from the commonly used Hen egg white lysozyme (HEWL). The enzyme has high activity against bacteria of the genus Thermus including Thermusthermophilus and Thermus aquaticus as shown in the Table:

 Bacterial species Ts Lysozyme  HEWL
 Thermus thermophilus HB8  100  43
 Thermus aquaticus  100  41
 Deinococcus radiodurans  25  21
 Echerichia coli  34  100
 Salmonella panama  10  35
 Pseudomonas fluorescence  13  40
 Serratia marcescens  0  35
 Enterococcus faecalis  4  0
 Bacillus subtilis  2  0
 Bacillus cereus  15  75
 Staphylococcus aureus  0  0
 Staphylococcus intermedius  0  5
 Staphylococcus epidermis  0  0
 Sarcina lutea  2  48
 Streptococcus pyogenes  0  11
 Lactococcus lactis  0  11

 

 Relative activity (%): ThermoPhage™ Lysozyme vs. Hen Egg White Lysozyme
Lys164 Lysozyme activity vs.
Temperature profile: The enzyme has >80% activity between 40°and 100°C

 

 

 

Lys164 Lysozyme activity vs. pH

  pH optimum: the enzyme has optimum activity around pH  7.5 – 8

 Activity of Lys164 Lysozyme vs HEW Lysozyme

Thermostability: Relative activity of ThermoPhage™ Lysozyme at different temperature vs. the activity of Hen Egg White Lysozyme

 

 

Applications: The enzyme is suitable for efficient lysis of Thermus bacteria such as for increasing total DNA or plasmid DNA isolation (See figure below). Reactions and enzyme dilutions can be done in a 10 mM K/PO4 buffer at pH 8.0. The use of EDTA is not recommended.

Lys164-figure3b

DNA isolation: ThermoPhage™ Lysozyme improves plasmid preparation from Thermus thermophilus HB8

 

References: 

Plotka M, Stefanska A, Kaczorowska A-K, Morzywolek A, Fridjonsson OF, Dunin-Horkawicz S, Hreggvidsson GO, Kristjansson JK, Dabrowski S, Bujnicki JM and Kaczorowski T. 2014 Purification and characterization of a novel highly thermostable lysozyme fromThermus scotoductus MAT2119 bacteriophage Ph2119 that shows amino-acid sequence similarity to eukaryotic peptidoglycan recognition proteins (PGRPs). Appl. Environ. Microbiol.

Additional information

Unit size and price

0.1 mg – EUR 220