A thermostable 4-α-glucanotransferase that elongates linear alpha-glucan chains in starch and amylose by catalyzing the transfer of one glucose unit from the non-reducing end to a new position in an acceptor, which may be triose or larger. The enzyme can be used various applications, such as for modifications of alpha-polysaccharides and is ideal for making clear size ladders of oligosaccharides larger than maltose. The enzyme was developed from metagenome DNA obtained from environmental sample from a geothermal environment.
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Mode of action: The enzyme catalyzes the formation of the alpha-1,4-glucosidic linkages. The enzyme transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Unit Definition: Half unit (U) is defined as the amount of enzyme which lowers the OD at 650 nm from 1.2 to 0.7 in 30minutes.
Unit Assay Conditions: Branching enzyme activity was routinely determined in a 50mM KPO4 pH 8.0 buffer for 10 minutes, with 0.01% (w/v) final conc. of amylose.
Source: Bacterial Environmental DNA
Temperature Optimum: The enzyme in relatively active in a rather broad temperature range with optimum around 70°C (Figure 1)
pH Optimum: pH optimum is around 7 (Figure 2)