A thermostable β-galactosidase that hydrolyses terminal, non-reducing β-D-galactose residues in β-D-galactosides. From the thermophilic bacterium Thermus brockianus.
Available as 1000 units. Shipping within a week, shipping and handling charges additional to price below.
Product : Bgal112 | Beta-Galactosidase 1000 Units | Price : 220.00 EUR
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Mode of action: The enzyme hydrolyses terminal, non-reducing b-D-galactose residues in b-D-galactosides.
Unit Definition: One unit (U) is defined as the amount of enzyme which liberates 1 mmol of p-nitrophenol per min from pNP-b-galactoside, under the given assay conditions.
Unit Assay Conditions: The standard assay for cellulase activity was done by incubating the enzyme at 80°C for 10 min, with 1 % (w/v) pustulan, as a substrate in 0.1M sodium phosphate buffer, pH 5,5. The reducing sugars released were detected by the dinitrosalicylic acid method using glucose as standard.
Temperature Optimum: The enzyme activity is limited below 65°C but increases with temperature up to 100°C
pH Optimum: pH range is about 4-7 with optimum around 6.5
Thermostability: The ß-galactosidase heat stability at 70,80 and 91°C