Alpha-Fucosidase from environmental DNA

fuc123

220,00

Thermostable Alpha-L-Fucosidase that cleaves alpha-glucan residues from various alpha-fucosylated oligosaccharides.


SKU: fuc123.
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Description

fuc123Fuc123 Alpha-L-Fucosidase is thermostable enzyme that cleaves alpha-glucan residues from various alpha-fucosylated oligosaccharides including hydrolysis of alpha-L-fucose from algal fucosidan (Ascophyllum nodosum).

The enzyme is available for purchase as 30 units of enzyme.

Shipping within a week, shipping and handling charges additional to price listed below.

Product : Fucl123 | Fucosidase 30 units | Price : 220.00 EUR

For further information read more below or download the product sheet

 

 

Enzyme activity: Fuc123 Alpha-Fucosidase is a glycosyl hydrolase that cleaves alpha-glucan residues from various alpha-fucosylated oligosaccharides including hydrolysis of alpha-L-fucose from algal fucosidan (Ascophyllum nodosum).

Activity determination: The standard assay is performed by mixing 10 μL enzyme sample to 80 μL of 125mM phosphate buffer at pH 6,5 with 20 μL PNP-alpha-fucopyranoside, incubated at 65°C. Reaction is stopped with 200μL 0,5M sodium borat and  OD measured at 405 nm. 

 

Unit definition: One unit of activity is defined as the amount of enzyme, which liberates 1 μmol of p-nitrophenol per minute under the given assay conditions.

 

Synonyms: glycoside hydrolase, alpha-L-fucoside fucohydrolase, alpha-fucosidase

Protein family: Glycosyl hydrolase family

 

Enzyme classificationEC 3.2.1.51

 

Source: Proprietary environmental DNA

 

Substrates:

Fucoidan is a group of sulfated polysaccharides containing fucose and includes molecules with a backbone built of (1→3)-linked alpha-L-fucopyranosyl or alternating (1→3)- and (1→4)-linked alpha-L-fucopyranosyl residues, but also includes sulfated galactofucans with backbones built of (1→6)-beta-D-galacto- and/or (1→2)-beta-D-mannopyranosyl units and may include branching and various substitutions of sugar residues. Fucoidan from seaweed has found use as dietary supplement and has been implicated as having potential bioactive functions in humans ( Ale et al 2011 ).

 

Properties of ThermoActive™ Alpha-Fucosidase:

 

Fuc123 Fucosidase activity vs. temperature

Temperature Optimum: The enzyme in relatively active in a temperature range (60-70°C)with optimum around 65°C

 

Fuc123 Fucosidase activity vs. pH

pH Optimum: suitable pH range is about 6-7 with optimum around 6.5

 

References:

Ale MT, Mikkelsen JD & Meyer AS (2011). Important Determinants for Fucoidan Bioactivity: A Critical Review of Structure-Function Relations and Extraction Methods for Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds. Marine Drugs 9: 2106–2130

Additional information

Unit size and price

30 units – EUR 220