Alcohol dehydrogenase from Geothermus vaporicella

adh131

220,00

A thermostable  alcohol dehydrogenase. Most active on n-butanol but low activity on ethanol.


SKU: adh131.
Category: .

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Description

adh131A thermostable  alcohol dehydrogenase. Most active on n-butanol but low activity on ethanol.                         

 

Available as 100 units. Shipping within a week, shipping and handling charges additional to price listed below.

Product : Adh131 | Alcohol dehydrogenase 100 Units | Price : 220.00 EUR

 

 

For further information read more below or download the product sheet 

 

Mode of action: The enzyme is an alcohol dehydrogenase most active on n-butanol but with low activity on ethanol.

Unit Definition: One unit (U) of enzyme activity is defined as the absorbance measured after incubation for 10 min at 340nm (NAD+ turning into NADH) units (U) are defined as mmol/min of product released.

Adh131 Alcohol dehydrogenase activity vs. temperature

Temperature Optimum: The enzyme in relatively active at broad temperature range (50-65°C)with optimum around 60°C.

Adh131 Alcohol dehydrogenase activity vs. pHpH Optimum: pH range is about 8-9 with optimum around  pH 8,0.

 

Adh131 Alcohol dehydrogenase thermostabilityThermostability: The enzyme is stable over 400 minutes at temperature at 65°C.

Unit Assay Conditions: 

100 µl sample:

 

  • 100 µl 1-butanol (110mM)
  • 200 µl NAD+  (50mM)
  • 100 µl KPO4 pH 8 (500mM)
  • 10 µl zinksalt  (100mM)
  • 390 µl dH2O

 

Incubated at 60°C for 10min.  Reaction stopped on ice.  Spin shortly at 4°C before measuring the absorbance at 340nm.

Additional information

Unit size and price

100 units – EUR 220